# name
A humanized mouse model for sequestration of Plasmodium
                falciparum sexual stages and in vivo evaluation of gametocytidal
                drugs

# metadata.dc.description.abstract[].value
The development of new drugs to disrupt malaria transmission
                requires the establishment of an in vivo model to address the
                biology of Plasmodium falciparum sexual stages (gametocytes).
                Herein we show that chemically immune-modulated NSG mice grafted
                with human erythrocytes support complete sexual development of
                P. falciparum parasites and generate high gametocytemia.
                Immunohistochemistry and RT-qPCR analyses indicate an enrichment
                of immature gametocytes in the bone marrow and the spleen,
                suggesting a sequestration mechanism reminiscent to that
                observed in humans. Upon primaquine treatment, elimination of
                gametocytes from peripheral blood and from sequestration sites
                was observed, providing a proof of concept that these mice can
                be used for testing drugs. Therefore, this model allows the
                investigation of P. falciparum sexual commitment, gametocyte
                interactions with the bone marrow and spleen and provides the
                missing link between current in vitro assays and Phase I trials
                in humans for testing new malaria gametocytidal drugs.

# metadata.dc.relation.isformatof[].value
Reproducció del document publicat a:
                http://dx.doi.org/10.1038/srep35025

# metadata.dc.source[].value
Articles publicats en revistes (ISGlobal)

# metadata.dc.title[].value
A humanized mouse model for sequestration of Plasmodium
                falciparum sexual stages and in vivo evaluation of gametocytidal
                drugs