# name
First insights into the pleiotropic role of vrf (yedF), a newly
                characterized gene of Salmonella Typhimurium

# metadata.dc.description.abstract[].value
Salmonella possesses virulence determinants that allow
                replication under extreme conditions and invasion of host cells,
                causing disease. Here, we examined four putative genes predicted
                to encode membrane proteins (ydiY, ybdJ, STM1441 and ynaJ) and a
                putative transcriptional factor (yedF). These genes were
                identified in a previous study of a S. Typhimurium clinical
                isolate and its multidrug-resistant counterpart. For STM1441 and
                yedF a reduced ability to interact with HeLa cells was observed
                in the knock-out mutants, but an increase in this ability was
                absent when these genes were overexpressed, except for yedF
                which phenotype was rescued when yedF was restored. In the
                absence of yedF, decreased expression was seen for: i)
                virulence-related genes involved in motility, chemotaxis,
                attachment and survival inside the host cell; ii) global
                regulators of the invasion process (hilA, hilC and hilD); and
                iii) factors involved in LPS biosynthesis. In contrast, an
                increased expression was observed for anaerobic metabolism
                genes. We propose yedF is involved in the regulation of
                Salmonella pathogenesis and contributes to the activation of the
                virulence machinery. Moreover, we propose that, when oxygen is
                available, yedF contributes sustained repression of the
                anaerobic pathway. Therefore, we recommend this gene be named
                vrf, for virulence-related factor.

# metadata.dc.relation.ispartof[].value
Scientific Reports, 2017, vol. 7, num. 15291

# metadata.dc.source[].value
Articles publicats en revistes (ISGlobal)

# metadata.dc.title[].value
First insights into the pleiotropic role of vrf (yedF), a newly
                characterized gene of Salmonella Typhimurium