# name
Gametocytes of the Malaria Parasite Plasmodium falciparum
                Interact With and Stimulate Bone Marrow Mesenchymal Cells to
                Secrete Angiogenetic Factors

# metadata.dc.description.abstract[].value
The gametocytes of Plasmodium falciparum, responsible for the
                transmission of this malaria parasite from humans to mosquitoes,
                accumulate and mature preferentially in the human bone marrow.
                In the 10 day long sexual development of P. falciparum, the
                immature gametocytes reach and localize in the extravascular
                compartment of this organ, in contact with several bone marrow
                stroma cell types, prior to traversing the endothelial lining
                and re-entering in circulation at maturity. To investigate the
                host parasite interplay underlying this still obscure process,
                we developed an in vitro tridimensional co-culture system in a
                Matrigel scaffold with P. falciparum gametocytes and
                self-assembling spheroids of human bone marrow mesenchymal cells
                (hBM-MSCs). Here we show that this co-culture system sustains
                the full maturation of the gametocytes and that the immature,
                but not the mature, gametocytes adhere to hBM-MSCs via
                trypsin-sensitive parasite ligands exposed on the erythrocyte
                surface. Analysis of a time course of gametocytogenesis in the
                co-culture system revealed that gametocyte maturation is
                accompanied by the parasite induced stimulation of hBM-MSCs to
                secrete a panel of 14 cytokines and growth factors, 13 of which
                have been described to play a role in angiogenesis. Functional
                in vitro assays on human bone marrow endothelial cells showed
                that supernatants from the gametocyte mesenchymal cell
                co-culture system enhance ability of endothelial cells to form
                vascular tubes. These results altogether suggest that the
                interplay between immature gametocytes and hBM-MSCs may induce
                functional and structural alterations in the endothelial lining
                of the human bone marrow hosting the P. falciparum transmission
                stages.

# metadata.dc.relation.isformatof[].value
Reproducció del document publicat a:
                http://dx.doi.org/10.3389/fcimb.2018.00050

# metadata.dc.relation.ispartof[].value
Frontiers in Cellular and Infection Microbiology, 2018, vol. 8, num. 50

# metadata.dc.source[].value
Articles publicats en revistes (ISGlobal)

# metadata.dc.title[].value
Gametocytes of the Malaria Parasite Plasmodium falciparum
                Interact With and Stimulate Bone Marrow Mesenchymal Cells to
                Secrete Angiogenetic Factors