# name
Congenital cytomegalovirus, parvovirus and enterovirus infection
                in Mozambican newborns at birth: A cross-sectional survey

# metadata.dc.description.abstract[].value
BACKGROUND: Congenital cytomegalovirus (cCMV) infection is the
                most prevalent congenital infection acquired worldwide, with
                higher incidence in developing countries and among HIV-exposed
                children. Less is known regarding vertical transmission of
                parvovirus B19 (B19V) and enterovirus (EV). We aimed to assess
                the prevalence of CMV, B19V and EV vertical transmission and
                compare results of screening of congenital CMV obtained from two
                different specimens in a semirural Mozambican maternity.
                METHODS: A cross sectional study was conducted among pregnant
                mothers attending Manhica District Hospital upon delivery.
                Information on maternal risk factors was ascertained. Dried
                umbilical cord (DUC) samples were collected in filter paper for
                CMV, B19V and EV detection by real-time polymerase chain
                reaction (RT-PCR), and nasopharyngeal aspirates (NPA) to test
                for CMV by RT-PCR. Maternal blood samples and placental biopsy
                samples were also obtained to investigate CMV maternal serology,
                HIV status and immunopathology. RESULTS: From September 2014 to
                January 2015, 118 mothers/newborn pairs were recruited.
                Prevalence of maternal HIV infection was 31.4% (37/118). CMV
                RT-PCR was positive in 3/115 (2.6%) of DUC samples and in 3/96
                (6.3%) of NPA samples obtained from neonates. The concordance of
                the RT-PCR assay through DUC with their correspondent NPA sample
                was moderate (Kappa = 0.42 and p<0.001. No differences on
                cCMV prevalence were found among HIV-exposed and unexposed. All
                (100%) mothers were seropositive for CMV IgG. RT-PCR of EV and
                B19V in DUC were both negative in all screened cases. No
                histological specific findings were found in placental tissues.
                No risk factors associated to vertical transmission of these
                viral infections were found. CONCLUSIONS: This study indicates
                the significant occurrence of vertical transmission of CMV in
                southern Mozambique. Larger studies are needed to evaluate the
                true burden, clinical relevance and consequences of congenital
                infections with such pathogens in resource-constrained settings.

# metadata.dc.relation.isformatof[].value
Reproducció del document publicat a:
                http://dx.doi.org/10.1371/journal.pone.0194186

# metadata.dc.source[].value
Articles publicats en revistes (ISGlobal)

# metadata.dc.title[].value
Congenital cytomegalovirus, parvovirus and enterovirus infection
                in Mozambican newborns at birth: A cross-sectional survey