# name
Genistein induces adipogenic and autophagic effects in rainbow trout (Oncorhynchus mykiss) adipose tissue: in vivo and in vitro models

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Soybeans are one of the most used alternative dietary ingredients in aquafeeds. However, they contain phytoestrogens like genistein (GE), which can have an impact on fish metabolism and health. This study aimed to investigate the in vitro and in vivo effects of GE on lipid metabolism, apoptosis, and autophagy in rainbow trout (Oncorhynchus mykiss). Primary cultured preadipocytes were incubated with GE at different concentrations, 10 or 100 μM, and 1 μM 17β-estradiol (E2). Furthermore, juveniles received an intraperitoneal injection of GE at 5 or 50 µg/g body weight, or E2 at 5 µg/g. In vitro, GE 100 μM increased lipid accumulation and reduced cell viability, apparently involving an autophagic process, indicated by the higher LC3-II protein levels, and higher lc3b and cathepsin d transcript levels achieved after GE 10 μM. In vivo, GE 50 µg/g upregulated the gene expression of fatty acid synthase (fas) and glyceraldehyde-3-phosphate dehydrogenase in adipose tissue, suggesting enhanced lipogenesis, whereas it increased hormone-sensitive lipase in liver, indicating a lipolytic response. Besides, autophagy-related genes increased in the tissues analyzed mainly after GE 50 µg/g treatment. Overall, these findings suggest that an elevated GE administration could lead to impaired adipocyte viability and lipid metabolism dysregulation in rainbow trout

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International Journal of Molecular Sciences, 2020, vol. 21, num. 16, p. 5884

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cc-by (c) Balbuena-Pecino, Sara et al., 2020

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Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)

# metadata.dc.title[].value
Genistein induces adipogenic and autophagic effects in rainbow trout (Oncorhynchus mykiss) adipose tissue: in vivo and in vitro models