# name
Reporter lines based on the gexp02 promoter enable early quantification of sexual conversion rates in the malaria parasite Plasmodium falciparum

# metadata.dc.description.abstract[].value
Transmission of malaria parasites from humans to mosquito
                vectors requires that some asexual parasites differentiate into
                sexual forms termed gametocytes. The balance between
                proliferation in the same host and conversion into transmission
                forms can be altered by the conditions of the environment. The
                ability to accurately measure the rate of sexual conversion
                under different conditions is essential for research addressing
                the mechanisms underlying sexual conversion, and to assess the
                impact of environmental factors. Here we describe new Plasmodium
                falciparum transgenic lines with genome-integrated constructs in
                which a fluorescent reporter is expressed under the control of
                the promoter of the gexp02 gene. Using these parasite lines, we
                developed a sexual conversion assay that shortens considerably
                the time needed for an accurate determination of sexual
                conversion rates, and dispenses the need to add chemicals to
                inhibit parasite replication. Furthermore, we demonstrate that
                gexp02 is expressed specifically in sexual parasites, with
                expression starting as early as the sexual ring stage, which
                makes it a candidate marker for circulating sexual rings in
                epidemiological studies.

# metadata.dc.source[].value
Articles publicats en revistes (ISGlobal)

# metadata.dc.title[].value
Reporter lines based on the gexp02 promoter enable early quantification of sexual conversion rates in the malaria parasite Plasmodium falciparum