# name
High frequency of the exoU+/exoS+ genotype associated with multidrug-resistant "high-risk clones" of Pseudomonas aeruginosa clinical isolates from Peruvian hospitals

# metadata.dc.description.abstract[].value
The type III secretion system of Pseudomonas aeruginosa
                is an important virulence factor contributing to the
                cytotoxicity and the invasion process of this microorganism. The
                current study aimed to determine the presence of the exoU+/exoS+
                genotype in P. aeruginosa clinical isolates. The presence of
                exoS, exoT, exoU and exoY was determined in 189 P. aeruginosa by
                PCR, and the presence/absence of exoU was analysed according to
                source infection, clonal relationships, biofilm formation,
                motility and antimicrobial susceptibility. The gyrA, parC, oprD,
                efflux pump regulators and \xCE\xB2-lactamases genes were also
                analysed by PCR/sequencing. The exoS, exoT and exoY genes were
                found in 100% of the isolates. Meanwhile, exoU was present in
                43/189 (22.8%) of the isolates, being significantly associated
                with multidrug resistance, extensively drug resistance as well
                as with higher level quinolone resistance. However, the presence
                of \xCE\xB2-lactamases, mutations in gyrA and parC, and relevant
                modifications in efflux pumps and OprD were not significantly
                associated with exoU+ isolates. MLST analysis of a subset of 25
                isolates showed 8 different STs displaying the exoU+/exoS+
                genotype. The MDR basis of the exoU+ isolates remain to be
                elucidated. Furthermore, the clinical implications and spread of
                exoU+/exoS+\xC2\xA0P. aeruginosa isolates need to be
                established.

# metadata.dc.source[].value
Articles publicats en revistes (ISGlobal)

# metadata.dc.title[].value
High frequency of the exoU+/exoS+ genotype associated with multidrug-resistant "high-risk clones" of Pseudomonas aeruginosa clinical isolates from Peruvian hospitals