# name
Immunogenic properties of Hepatitis A virus in two mouse strains. Preliminary results

# metadata.dc.description[].value
Treballs Finals de Grau de Farmàcia, Facultat de Farmàcia i Ciències de l'Alimentació, Universitat de Barcelona, 2021. Tutores: Malen Massot i Margarida Castell

# metadata.dc.description.abstract[].value
Hepatitis A (HA) is a viral disease caused by the hepatitis A virus (HAV) that occurs all
over the world. It is transmitted by the faecal–oral route, and it is known that poor sanitary
conditions increase the risk of HAV transmission.
As no specific treatment for HA is available, prevention with vaccination strategies is the
key to controlling the infection. Most of the available vaccines are inactivated vaccines
adjuvanted with aluminium hydroxide. The slow growth of HAV and the need for an
adjuvant make the vaccines’ economic cost relatively high and, what is more, there have
been periods of vaccine shortages as demand has exceeded the production capacity.
Recently, it has been demonstrated that HAV can be found encapsulated in a lipid
membrane that resembles exosomes. Exosomes have been suggested to take part in Tcell
stimulation and can induce an immune response without the need of an adjuvant.
This study includes the preliminary experiments that will conclude with immunization with
encapsulated HAV (eHAV) without adjuvant. In particular, herein we set up techniques
to check an immunization protocol with HAV particles (with adjuvant) in two mouse
strains (BALB/c and C75BL/6). These techniques include the proliferative capacity of
spleen lymphocytes (ELISA), the antibody-secreting cells (ELISPOT) and the changes
in the cell phenotype (flow cytometry). Although further studies remain to be done, the
results obtained in the present study allow the defining of the mouse strain and obtention
techniques to establish the immunization process.

# metadata.dc.source[].value
Treballs Finals de Grau (TFG) - Farmàcia

# metadata.dc.title[].value
Immunogenic properties of Hepatitis A virus in two mouse strains. Preliminary results