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Concentration methods for the quantification of coronavirus and other potentially pandemic enveloped virus from wastewater

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As the novel SARS-CoV-2 was detected in faeces, environmental researchers have been using centrifugal ultrafiltration, polyethylene glycol precipitation and aluminium hydroxide flocculation to describe its presence in wastewater samples. High recoveries (up to 65%) are described with electronegative filtration when using surrogate viruses, but few literature reports recovery efficiencies using accurate quantification of enveloped viruses. Considering that every single virus will have a different behaviour during viral concentration, it is recommended to use an enveloped virus, and if possible, a betacoronaviruses as murine hepatitis virus, as a surrogate. In this review, we show new data from a newly available technology that provides a quick ultrafiltration protocol for SARSCoV- 2. Wastewater surveillance is an efficient system for the evaluation of the relative prevalence of SARS-CoV-2 infections in a community, and there is the need of using reliable concentration methods for an accurate and sensitive quantification of the virus in water.

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Current Opinion in Environmental Science & Health, 2020, vol. 17, p. 21-28

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Articles publicats en revistes (Genètica, Microbiologia i Estadística)

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Concentration methods for the quantification of coronavirus and other potentially pandemic enveloped virus from wastewater