# name
Development of a genetic tool for functional screening of
                anti-malarial bioactive extracts in metagenomic libraries

# metadata.dc.description.abstract[].value
BACKGROUND: The chemical treatment of Plasmodium falciparum for
                human infections is losing efficacy each year due to the rise of
                resistance. One possible strategy to find novel anti-malarial
                drugs is to access the largest reservoir of genomic biodiversity
                source on earth present in metagenomes of environmental
                microbial communities. METHODS: A bioluminescent P. falciparum
                parasite was used to quickly detect shifts in viability of
                microcultures grown in 96-well plates. A synthetic gene encoding
                the Dermaseptin 4 peptide was designed and cloned under tight
                transcriptional control in a large metagenomic insert context
                (30 kb) to serve as proof-of-principle for the screening
                platform. RESULTS: Decrease in parasite viability consistently
                correlated with bioluminescence emitted from parasite
                microcultures, after their exposure to bacterial extracts
                containing a plasmid or fosmid engineered to encode the
                Dermaseptin 4 anti-malarial peptide. CONCLUSIONS: Here, a new
                technical platform to access the anti-malarial potential in
                microbial environmental metagenomes has been developed.

# metadata.dc.source[].value
Articles publicats en revistes (ISGlobal)

# metadata.dc.subject.classification[].value
Efecte dels medicaments sobre els microorganismes

# metadata.dc.title[].value
Development of a genetic tool for functional screening of
                anti-malarial bioactive extracts in metagenomic libraries