# name
Quantification of malaria antigens PfHRP2 and pLDH by
                quantitative suspension array technology in whole blood, dried
                blood spot and plasma

# metadata.dc.description.abstract[].value
Background: Malaria diagnostics by rapid diagnostic test (RDT) relies primarily on the qualitative detection of Plasmodium falciparum histidine-rich protein 2 (PfHRP2) and Plasmodium spp lactate dehydrogenase (pLDH). As novel
RDTs with increased sensitivity are being developed and implemented as point of care diagnostics, highly sensitive
laboratory-based assays are needed for evaluating RDT performance. Here, a quantitative suspension array technol‑
ogy (qSAT) was developed, validated and applied for the simultaneous detection of PfHRP2 and pLDH in a variety of
biological samples (whole blood, plasma and dried blood spots) from individuals living in diferent endemic countries.
Results: The qSAT was specifc for the target antigens, with analytical ranges of 6.8 to 762.8 pg/ml for PfHRP2 and
78.1 to 17076.6 pg/ml for P. falciparum LDH (Pf-LDH). The assay detected Plasmodium vivax LDH (Pv-LDH) at a lower
sensitivity than Pf-LDH (analytical range of 1093.20 to 187288.5 pg/ml). Both PfHRP2 and pLDH levels determined
using the qSAT showed to positively correlate with parasite densities determined by quantitative PCR (Spearman
r=0.59 and 0.75, respectively) as well as microscopy (Spearman r=0.40 and 0.75, respectively), suggesting the assay
to be a good predictor of parasite density.
Conclusion: This immunoassay can be used as a reference test for the detection and quantifcation of PfHRP2 and
pLDH, and could serve for external validation of RDT performance, to determine antigen persistence after parasite
clearance, as well as a complementary tool to assess malaria burden in endemic settings.

# metadata.dc.rights[].value
cc by (c) Martiáñez Vendrell et al., 2020

# metadata.dc.source[].value
Articles publicats en revistes (ISGlobal)

# metadata.dc.title[].value
Quantification of malaria antigens PfHRP2 and pLDH by
                quantitative suspension array technology in whole blood, dried
                blood spot and plasma