# name
Succinyl-CoA Synthetase: New Antigen Candidate of Bartonella
                bacilliformis

# metadata.dc.description.abstract[].value
BACKGROUND: Bartonella bacilliformis is the causative agent of
                Carrion's disease, a neglected illness with mortality rates of
                40-85% in the absence of treatment. The lack of a diagnostic
                technique to overcome misdiagnosis and treat asymptomatic
                carriers is of note. This study aimed to identify new B.
                bacilliformis antigenic candidates that could lead to a new
                diagnostic tool able to be implemented in endemic rural areas.
                METHODOLOGY/PRINCIPAL FINDINGS: Blood (n = 198) and serum (n =
                177) samples were collected in northern Peru. Clinical data were
                recorded. Specific 16S rRNA amplification by RT-PCR, IFA and
                ELISA for IgM/IgG with whole cells as antigens was done. Western
                blot analysis and N-terminal amino acid sequencing detected
                seroreactive proteins. ELISAs for IgM/IgG for the antigenic
                candidates were performed. Of the population 33.3% reported at
                least one symptom compatible with Carrion's disease; 25.4%
                (IFA), 27.1% (ELISA-IgG), 33.9% (ELISA-IgM) and 38.9% (RT-PCR)
                of samples were positive. Four proteins were considered
                potential antigenic candidates, including two new antigenic
                candidates, succinyl-CoA synthetase subunit alpha (SCS-alpha)
                and succinyl-CoA synthetase subunit beta (SCS-beta). On Western
                blot both Pap31 and SCS-alpha interacted with IgM, while GroEL
                and SCS-beta interacted with IgG. The presence of specific
                antibodies against the antigenic candidates varied from 34.5%
                (IgG against SCS-alpha) to 97.2% (IgM against Pap31).
                CONCLUSIONS/SIGNIFICANCE: RT-PCR and the high levels of
                positivity for specific ELISAs demonstrate high levels of B.
                bacilliformis exposure and asymptomatic carriers among
                inhabitants. The new antigens identified might be used as a new
                rapid diagnostic tool to diagnose acute Carrion's disease and
                identify asymptomatic carriers.

# metadata.dc.relation.isformatof[].value
Reproducció del document publicat a:
                http://dx.doi.org/10.1371/journal.pntd.0004989

# metadata.dc.relation.ispartof[].value
PLoS Neglected Tropical Diseases, 2016, vol. 10, num. 9, p.
                e0004989

# metadata.dc.source[].value
Articles publicats en revistes (ISGlobal)

# metadata.dc.title[].value
Succinyl-CoA Synthetase: New Antigen Candidate of Bartonella
                bacilliformis