# name
The antigen-binding fragment of human gamma immunoglobulin
                prevents amyloid beta-peptide folding into beta-sheet to form
                oligomers

# metadata.dc.description.abstract[].value
The amyloid beta-peptide (Abeta) plays a leading role in
                Alzheimer's disease (AD) physiopathology. Even though monomeric
                forms of Abeta are harmless to cells, Abeta can aggregate into
                beta-sheet oligomers and fibrils, which are both neurotoxic.
                Therefore, one of the main therapeutic approaches to cure or
                delay AD onset and progression is targeting Abeta aggregation.
                In the present study, we show that a pool of human gamma
                immunoglobulins (IgG) protected cortical neurons from the
                challenge with Abeta oligomers, as assayed by MTT reduction,
                caspase-3 activation and cytoskeleton integrity. In addition, we
                report the inhibitory effect of IgG on Abeta aggregation, as
                shown by Thioflavin T assay, size exclusion chromatography and
                atomic force microscopy. Similar results were obtained with
                Palivizumab, a human anti-sincitial virus antibody. In order to
                dissect the important domains, we cleaved the pool of human IgG
                with papain to obtain Fab and Fc fragments. Using these cleaved
                fragments, we functionally identified Fab as the immunoglobulin
                fragment inhibiting Abeta aggregation, a result that was further
                confirmed by an in silico structural model. Interestingly,
                bioinformatic tools show a highly conserved structure able to
                bind amyloid in the Fab region. Overall, our data strongly
                support the inhibitory effect of human IgG on Abeta aggregation
                and its neuroprotective role.

# metadata.dc.relation.isformatof[].value
Reproducció del document publicat a:
                http://dx.doi.org/10.18632/oncotarget.17074

# metadata.dc.source[].value
Articles publicats en revistes (ISGlobal)

# metadata.dc.title[].value
The antigen-binding fragment of human gamma immunoglobulin
                prevents amyloid beta-peptide folding into beta-sheet to form
                oligomers