# name
Artilysation' of endolysin λSa2lys strongly improves its enzymatic and antibacterial activity against streptococci

# metadata.dc.description.abstract[].value
Endolysins constitute a promising class of antibacterials against Gram-positive bacteria. Recently, endolysins have been engineered with selected peptides to obtain a new generation of lytic proteins, Artilysins, with specific activity against Gram-negative bacteria. Here, we demonstrate that artilysation can also be used to enhance the antibacterial activity of endolysins against Gram-positive bacteria and to reduce the dependence on external conditions. Art-240, a chimeric protein of the anti-streptococcal endolysin λSa2lys and the polycationic peptide PCNP, shows a similar species specificity as the parental endolysin, but the bactericidal activity against streptococci increases and is less affected by elevated NaCl concentrations and pH variations. Time-kill experiments and time-lapse microscopy demonstrate that the killing rate of Art-240 is approximately two-fold higher compared to wildtype endolysin λSa2lys, with a reduction in viable bacteria of 3 log units after 10min. In addition, lower doses of Art240 are required to achieve the same bactericidal effect.

# metadata.dc.relation.ispartof[].value
Scientific Reports, 2016, vol. 6, num. 35382

# metadata.dc.rights[].value
cc-by (c) Rodriguez Rubio, Lorena et al., 2016

# metadata.dc.source[].value
Articles publicats en revistes (Genètica, Microbiologia i Estadística)

# metadata.dc.title[].value
Artilysation' of endolysin λSa2lys strongly improves its enzymatic and antibacterial activity against streptococci