# name
A cytokine pattern that differentiates pre- from post-
                seroconversion phases of primary HIV infection

# metadata.dc.description.abstract[].value
During acute HIV infection (AHI) HIV actively replicates but
                seroconversion has not yet occurred. Primary HIV infection (PHI)
                is characterized by a transient non-specific febrile illness, a
                massive inflammatory response and the progressive appearance of
                anti-HIV specific antibodies. In this study we have identified
                patterns of inflammatory biomarkers associated with the innate
                immunological reaction prior to completion of a full humoral
                response. A symptom-based screening was used to identify AHI in
                the Manhica District Hospital in Mozambique. Plasma levels of
                biomarkers were determined by luminex and ELISA. Anti-HIV
                antibodies were analysed by flow-cytometry and Western blot.
                Statistical analyses used Random Forest and logistic regression
                models. Of 3116 rapid test seronegative or indeterminate
                individuals, 85 (2.7%) had positive plasma HIV viral load and
                were enrolled as PHI, of which n=45 (52.9%), n=8 (9.4%), n=12
                (14.1%) and n=20 (23.5%) were classified as Fiebig I-III, IV, V
                and VI stages, respectively by Western-blot. Comparison of
                individuals at early (Fiebig I-IV) and late (Fiebig V-VI) immune
                stages identified significant differences in the expression
                level of plasma BAFF, MCP-1, sCD163 and MIG. This cytokine
                signature classified patients in the pre-seroconversion phase
                with a sensitivity of 92.5% and a specificity of 81.2%
                CONCLUSIONS:: Identification of a cytokine signature specific
                for the pre-seroconversion stage of PHI may help to understand
                the earliest HIV pathogenic events and identify new potential
                targets for immunotherapy aimed at modulating the cytokine
                response to HIV infection.This is an open-access article
                distributed under the terms of the Creative Commons
                Attribution-Non Commercial-No Derivatives License 4.0
                (CCBY-NC-ND), where it is permissible to download and share the
                work provided it is properly cited. The work cannot be changed
                in any way or used commercially without permission from the
                journal.

# metadata.dc.relation.ispartof[].value
Journal of Acquired Immune Deficiency Syndromes, 2017, vol. 74, num. 4, p. 459-466

# metadata.dc.source[].value
Articles publicats en revistes (ISGlobal)

# metadata.dc.title[].value
A cytokine pattern that differentiates pre- from post-
                seroconversion phases of primary HIV infection