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Biotechnological production of taxanes: a molecular approach

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Podeu consultar el llibre complet a: http://hdl.handle.net/2445/46988

# metadata.dc.description.abstract[].value
Plant cell cultures constitute a promise for the production of a high number of phytochemicals, although the majority of
bioprocesses that have been developed so far have not resulted
commercially successful. An overview indicates that most of the
research carried out until now is of the empirical type. For this reason,
there is a need for a rational approach to the molecular and cellular
basis of metabolic pathways and their regulation in order to stimulate
future advances.
The empirical investigations are based on the optimization of the
culture system, exclusively considering input factors such as the
selection of cellular lines, type and parameters of culture, bioreactor
design and elicitor addition, and output factors such as cellular growth,
the uptake system of nutrients, production and yield. In a rational
approach towards the elucidation of taxol and related taxane
production, our group has studied the relationship between the taxane
profile and production and the expression of genes codifying for
enzymes that participate in early, intermediate and late steps of their
biosynthesis in elicited Taxus spp cell cultures. Our results show that elicitors induce a dramatic reprogramming of gene expression in Taxus cell cultures, which
likely accounts for the enhanced production of taxol and related taxanes and we have also
determined some genes that control the main flux limiting steps. The application of
metabolic engineering techniques for the production of taxol and taxanes of interest is also discussed.

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Recent Advances in Pharmaceutical Sciences III, 2013, Transworld Research Network : Editors: Diego Muñoz Torrero, Amparo Cortés & Eduardo L. Mariño. ISBN: 978-81-7895-605-3. Chapter 6, p. 91-107.

# metadata.dc.source[].value
Llibres / Capítols de llibre (Biologia, Sanitat i Medi Ambient)

# metadata.dc.title[].value
Biotechnological production of taxanes: a molecular approach