# name
Protocols for the evaluation of neurodevelopmental alterations in rabbit models in vitro and in vivo.

# metadata.dc.description.abstract[].value
The rabbit model is gaining importance in the field of neurodevelopmental evaluation due to
its higher similarity to humans in terms of brain development and maturation than rodents.
In this publication, we detailed 14 protocols covering toxicological relevant endpoints for
the assessment of neurodevelopmental adverse effects in the rabbit species. These
protocols include both in vitro and in vivo techniques, which also cover different
evaluation time-points, the neonatal period, and long-term examinations at postnatal
days (PNDs) 50–70. Specifically, the protocols (P) included are as follows: neurosphere
preparation (GD30/PND0; P2) and neurosphere assay (P3), behavioral ontogeny (PND1;
P4), brain obtaining and brain weight measurement at two different ages: PND1 (P5) and
PND70 (P12), neurohistopathological evaluations after immersion fixation for neurons,
astrocytes, oligodendrocytes and microglia (PND1; P6-9) or perfusion fixation (PND70;
P12), motor activity (P11, open field), memory and sensory function (P11, object
recognition test), learning (P10, Skinner box), and histological evaluation of plasticity
(P13 and P14) through dendritic spines and perineuronal nets. The expected control
values and their variabilities are presented together with the information on how to
troubleshoot the most common issues related to each protocol. To sum up, this
publication offers a comprehensive compilation of reliable protocols adapted to the
rabbit model for neurodevelopmental assessment in toxicology.

# metadata.dc.source[].value
Articles publicats en revistes (Cirurgia i Especialitats Medicoquirúrgiques)

# metadata.dc.title[].value
Protocols for the evaluation of neurodevelopmental alterations in rabbit models in vitro and in vivo.