# name
A Plasmodium falciparum C-mannosyltransferase is dispensable for parasite asexual blood stage development

# metadata.dc.description.abstract[].value
C-mannosylation was recently identified in the thrombospondin-related anonymous protein
(TRAP) from Plasmodium falciparum salivary gland sporozoites. A candidate P. falciparum
C-mannosyltransferase (Pf DPY-19) was demonstrated to modify thrombospondin type 1
repeat (TSR) domains in vitro, exhibiting a different acceptor specificity than their mammalian counterparts. According to the described minimal acceptor of Pf DPY19, several TSR
domain-containing proteins of P. falciparum could be C-mannosylated in vivo. However,
the relevance of this protein modification for the parasite viability remains unknown. In
the present study, we used CRISPR/Cas9 technology to generate a Pf DPY19 null mutant,
demonstrating that this glycosyltransferase is not essential for the asexual blood development
of the parasite. Pf DPY19 gene disruption was not associated with a growth phenotype, not
even under endoplasmic reticulum-stressing conditions that could impair protein folding.
The data presented in this work strongly suggest that Pf DPY19 is unlikely to play a critical
role in the asexual blood stages of the parasite, at least under in vitro conditions.

# metadata.dc.rights[].value
cc by (c) Cambridge University Press, 2019

# metadata.dc.source[].value
Articles publicats en revistes (ISGlobal)

# metadata.dc.title[].value
A Plasmodium falciparum C-mannosyltransferase is dispensable for parasite asexual blood stage development