Isoprenoid biosynthesis in Escherichia coli via the methylerythritol phosphate pathway: enzymatic conversion of methylerythritol cyclodiphosphate into a phosphorylated derivative of (E)-2-methylbut-2-ene-1,4-diol

Seemann, MyriamCampos Martínez, NarcisoRodríguez Concepción, ManuelIbañez, EstherDuvold, ToreTritsch, DenisBoronat i Margosa, AlbertRohmer, Michel2026-01-222026-01-222002-02-180040-4039https://hdl.handle.net/2445/225926A crude cell-free system from an Escherichia coli strain overexpressing the cluster containing the three genes yfgA, yfgB, and gcpE converted 2-C-methyl-d-erythritol 2,4-cyclodiphosphate (1) into a phosphorylated derivative of (E)-2-methylbut-2-ene-1,4-diol (6), which most probably represents a novel intermediate in the methylerythritol phosphate pathway for isoprenoid biosynthesis. The free diol 6 was accumulated by phosphatase treatment of the crude enzyme preparation and was identified by comparison with a synthetic reference.3 p.application/pdfeng(c) Elsevier Ltd., 2002Escheríchia coliEnzims microbiansEscherichia coliMicrobial enzymesIsoprenoid biosynthesis in Escherichia coli via the methylerythritol phosphate pathway: enzymatic conversion of methylerythritol cyclodiphosphate into a phosphorylated derivative of (E)-2-methylbut-2-ene-1,4-diolinfo:eu-repo/semantics/article5114782026-01-22info:eu-repo/semantics/openAccess