Fernández Bañares, FernandoLópez Palacios, NataliaCorzo, MaríaArau, BeatrizRubio, MercedesFernández Prieto, MartaTristán, EvaPujals, MarFarrais, SergioHorta, SáulHernández, Juana MariaGomez Perosanz, MartaReche, Pedro AntonioEsteve i Comas, MariaNúñez López, Concepción2022-05-272022-05-272021-10-061741-7015https://hdl.handle.net/2445/186104Background: The diagnosis of coeliac disease (CD) in individuals that have started a gluten-free diet (GFD) without an adequate previous diagnostic work-out is a challenge. Several immunological assays such as IFN-γ ELISPOT have been developed to avoid the need of prolonged gluten challenge to induce the intestinal damage. We aimed to evaluate the diagnostic accuracy of activated gut-homing CD8+ and TCRγδ+ T cells in blood after a 3-day gluten challenge and to compare it with the performance of IFN-γ ELISPOT in a HLA-DQ2.5 subsample. Methods: A total of 22 CD patients and 48 non-CD subjects, all of them following a GFD, underwent a 3-day 10-g gluten challenge. The percentage of two T cell subsets (CD8+ CD103+ β7hi CD38+/total CD8+ and TCRγδ+ CD103+ β7hi CD38+/total TCRγδ+) in fresh peripheral blood drawn baseline and 6 days after the challenge was determined by flow cytometry. IFN-γ ELISPOT assays were also performed in HLA-DQ2.5 participants. ROC curve analysis was used to assess the diagnostic performance of the CD8+ T cell response and IFN-γ ELISPOT. Results: Significant differences between the percentage of the two studied subsets of CD8+ and TCRγδ+ cells at days 0 and 6 were found only when considering CD patients (p < 10-3 vs. non-CD subjects). Measuring activated CD8+ T cells provided accurate CD diagnosis with 95% specificity and 97% sensitivity, offering similar results than IFN-γ ELISPOT. Conclusions: The results provide a highly accurate blood test for CD diagnosis in patients on a GFD of easy implementation in daily clinical practice.10 p.application/pdfengcc-by (c) Fernández Bañares, Fernando et al., 2021https://creativecommons.org/licenses/by/4.0/Malaltia celíacaAssaigs clínicsCitometria de fluxeDieta sense glutenCèl·lules TCeliac diseaseClinical trialsFlow cytometryGluten-free dietT cellsinfo:eu-repo/semantics/article7168262022-05-27info:eu-repo/semantics/openAccess34610833