Vázquez Fresno, RosaLlorach, RafaelAlcaro, FrancescaRodríguez Martínez, Miguel ÁngelVinaixa Crevillent, MariaChiva Blanch, GemmaEstruch Riba, RamonCorreig Blanchar, XavierAndrés Lacueva, Ma. Cristina2015-06-292015-06-292012-080173-0835https://hdl.handle.net/2445/66118Moderate wine consumption is associated with health-promoting activities. An H-NMR-based metabolomic approach was used to identify urinary metabolomic differences of moderate wine intake in the setting of a prospective, randomized, crossover, and controlled trial. Sixty-one male volunteers with high cardiovascular risk factors followed three dietary interventions (28 days): dealcoholized red wine (RWD) (272mL/day, polyphenol control), alcoholized red wine (RWA) (272mL/day) and gin (GIN) (100mL/day, alcohol control). After each period, 24-h urine samples were collected and analyzed by (1) H-NMR. According to the results of a one-way ANOVA, significant markers were grouped in four categories: alcohol-related markers (ethanol); gin-related markers; wine-related markers; and gut microbiota markers (hippurate and 4-hydroxphenylacetic acid). Wine metabolites were classified into two groups; first, metabolites of food metabolome: tartrate (RWA and RWD), ethanol, and mannitol (RWA); and second, biomarkers that relates to endogenous modifications after wine consumption, comprising branched-chain amino acid (BCAA) metabolite (3-methyl-oxovalerate). Additionally, a possible interaction between alcohol and gut-related biomarkers has been identified. To our knowledge, this is the first time that this approach has been applied in a nutritional intervention with red wine. The results show the capacity of this approach to obtain a comprehensive metabolome picture including food metabolome and endogenous biomarkers of moderate wine intake.10 p.application/pdfeng(c) Wiley-VCH, 2012ViConsum d'alcoholMetabolismeMalalties cardiovascularsRessonància magnètica nuclearMarcadors bioquímicsMetabòlitsWineDrinking of alcoholic beveragesMetabolismCardiovascular diseasesNuclear magnetic resonanceBiochemical markersMetabolitesinfo:eu-repo/semantics/article6182812015-06-29info:eu-repo/semantics/openAccess