Tarifa, CarmenVallmitjana, AlexanderJiménez Sábado, VerónicaMarchena, MiquelLlach, AnnaHerraiz Martínez, AdelaGodoy-Marín, HéctorNolla-Colomer, CarmeGinel, AntoninoViñolas, XavierMontiel, JoséCiruela Alférez, FranciscoEchebarria, BlasBenitez, RaúlCinca, JuanHove-Madsen, Leif2023-09-212023-09-212023-02-012452-302Xhttps://hdl.handle.net/2445/202167Analysis of the spatio-temporal distribution of calcium sparks showed a preferential increase in sparks near the sarcolemma in atrial myocytes from patients with atrial fibrillation (AF), linked to higher ryanodine receptor (RyR2) phosphorylation at s2808 and lower calsequestrin-2 levels. Mathematical modeling, incorporating modulation of RyR2 gating, showed that only the observed combinations of RyR2 phosphorylation and calsequestrin-2 levels can account for the spatio-temporal distribution of sparks in patients with and without AF. Furthermore, we demonstrate that preferential calcium release near the sarcolemma is key to a higher incidence and amplitude of afterdepolarizations in atrial myocytes from patients with AF15 p.application/pdfengcc-by (c) Tarifa, Carmen et al., 2023https://creativecommons.org/licenses/by/4.0/Fibril·lació auricularCèl·lules muscularsCanals de calciAtrial fibrillationMuscle cellsCalcium channelsinfo:eu-repo/semantics/article7323182023-09-21info:eu-repo/semantics/openAccess36777175