Sans de San Nicolàs, LídiaFigueras Nart, IgnasiGarcía-Jiménez, IreneBonfill Ortí, MontserratGuilabert, AntonioCurto Barredo, LaiaBertolín Colilla, MartaFerran, MartaSerra Baldrich, EstherPujol, Ramon M.Santamaria Babí, Luis F.2023-04-252023-04-252023-03-131664-3224https://hdl.handle.net/2445/197180Background:The role of allergen sensitization in IL-31 production by T cells and specifically in the clinical context of atopic dermatitis (AD) has not been characterized. MethodsThe response to house dust mite (HDM) in purified memory T cells cocultured with epidermal cells from AD patients (n=58) and control subjects (n=11) was evaluated. AD-associated cytokines from culture supernatants, plasma proteins and mRNA expression from cutaneous lesions were assessed and related with the clinical features of the patients. ResultsHDM-induced IL-31 production by memory T cells defined two subsets of AD patients according to the presence or absence of IL-31 response. Patients in the IL-31 producing group showed a more inflammatory profile, and increased HDM-specific (sp) and total IgE levels compared to the IL-31 non-producing group. A correlation between IL-31 production and patient's pruritus intensity, plasma CCL27 and periostin was detected. When the same patients were analyzed based on sp IgE and total IgE levels, an increased IL-31 in vitro response, as well as type 2 markers in plasma and cutaneous lesions, was found in patients with sp IgE levels > 100 kUA/L and total IgE levels > 1000 kU/L. The IL-31 response by memory T cells was restricted to the cutaneous lymphocyte-associated antigen (CLA)(+) T-cell subset. ConclusionIgE sensitization to HDM allows stratifying IL-31 production by memory T cells in AD patients and relating it to particular clinical phenotypes of the disease.12 p.application/pdfengcc by (c) Sans de San Nicolàs, Lídia et al., 2023http://creativecommons.org/licenses/by/3.0/es/Dermatitis atòpicaAl·lèrgensCitocinesCèl·lules TAtopic dermatitisAllergensCytokinesT cellsinfo:eu-repo/semantics/article2023-04-21info:eu-repo/semantics/openAccess36993985