Lister, RyanMukamel, Eran A.Nery, Joseph R.Urich, MarkPuddifoot, ClareJohnson, Nicholas D.Lucero, JacintaHuang, YunDwork, Andrew J.Schultz, Matthew D.Yu, MiaoTonti Filippini, JulianHeyn, HolgerHu, ShijunWu, Joseph C.Rao, AnjanaEsteller, Manel, 1968-He, ChuanHaghighi, Fatemeh GSejnowski, Terrence J.Behrens, M. MargaritaEcker, Joseph R.2018-11-122018-11-122013-08-090036-8075https://hdl.handle.net/2445/126021DNA methylation is implicated in mammalian brain development and plasticity underlying learning and memory. We report the genome-wide composition, patterning, cell specificity, and dynamics of DNA methylation at single-base resolution in human and mouse frontal cortex throughout their lifespan. Widespread methylome reconfiguration occurs during fetal to young adult development, coincident with synaptogenesis. During this period, highly conserved non-CG methylation (mCH) accumulates in neurons, but not glia, to become the dominant form of methylation in the human neuronal genome. Moreover, we found an mCH signature that identifies genes escaping X-chromosome inactivation. Last, whole-genome single-base resolution 5-hydroxymethylcytosine (hmC) maps revealed that hmC marks fetal brain cell genomes at putative regulatory regions that are CG-demethylated and activated in the adult brain and that CG demethylation at these hmC-poised loci depends on Tet2 activity.21 p.application/pdfeng(c) Lister, Ryan et al., 2013ADNAprenentatgeMemòriaDNALearningMemoryinfo:eu-repo/semantics/article6626952018-11-12info:eu-repo/semantics/openAccess23828890