Salort, José deCuenca, MartaTerhorst, CoxEngel Rocamora, PabloRomero Ros, Xavier2019-08-282019-08-282013-07-311664-3224https://hdl.handle.net/2445/138757The Signaling Lymphocyte Activation Molecule Family (SLAMF) genes, which encode cell-surface receptors that modulate innate and adaptive immune responses, lay within a genomic region of human and mouse chromosome 1 that confers a predisposition for the development of systemic lupus erythematosus (SLE). Herein, we demonstrate that the SLAMF member Ly9 arises as a novel receptor contributing to the reinforcement of tolerance. Specifically, Ly9-deficient mice spontaneously developed features of systemic autoimmunity such as the production of anti-nuclear antibodies (ANA), -dsDNA, and -nucleosome autoantibodies, independently of genetic background [(B6.129) or (BALB/c.129)]. In aged (10- to 12-month-old) Ly9 (-/-) mice key cell subsets implicated in autoimmunity were expanded, e.g., T follicular helper (Tfh) as well as germinal center (GC) B cells. More importantly, in vitro functional experiments showed that Ly9 acts as an inhibitory receptor of IFN-γ producing CD4(+) T cells. Taken together, our findings reveal that the Ly9 receptor triggers cell intrinsic safeguarding mechanisms to prevent a breach of tolerance, emerging as a new non-redundant inhibitory cell-surface receptor capable of disabling autoantibody responses.12 p.application/pdfengcc-by (c) Salort, Jose de et al., 2013http://creativecommons.org/licenses/by/3.0/esLupus eritematósAutoanticossosAntígensLupus erythematosusAutoantibodiesAntigensinfo:eu-repo/semantics/article6473802019-08-28info:eu-repo/semantics/openAccess23914190