Guillaumet Adkins, AmyRodríguez Esteban, GustavoMereu, ElisabettaMéndez Lago, MaríaJaitin, Diego A.Villanueva Garatachea, AlbertoVidal-Bel, AugustMartínez Martí, AlexFelip, EnriquetaVivancos, AnaKeren-Shaul, HadasHeath, Simon C.Gut, MartaAmit, IdoGut, Ivo G.Heyn, Holger2017-06-122017-06-122017-03-011474-7596https://hdl.handle.net/2445/112225A variety of single-cell RNA preparation procedures have been described. So far, protocols require fresh material, which hinders complex study designs. We describe a sample preservation method that maintains transcripts in viable single cells, allowing one to disconnect time and place of sampling from subsequent processing steps. We sequence single-cell transcriptomes from >1000 fresh and cryopreserved cells using 3'-end and full-length RNA preparation methods. Our results confirm that the conservation process did not alter transcriptional profiles. This substantially broadens the scope of applications in single-cell transcriptomics and could lead to a paradigm shift in future study designs.15 p.application/pdfengcc-by (c) Guillaumet-Adkins, Amy et al., 2017http://creativecommons.org/licenses/by/3.0/esRNACrioconservació d'òrgansCriobiologiaTranscripció genèticaGenòmicaCèl·lulesRNACryopreservation of organsCryobiologyGenetic transcriptionGenomicsCellsinfo:eu-repo/semantics/article6695742017-06-12info:eu-repo/semantics/openAccess28249587