Otify, Asmaa M.ElBanna, Shahira A.Eltanany, Basma M.Pont Villanueva, LauraBenavente Moreno, Fernando J. (Julián)Ibrahim, Rana M.2025-01-132025-01-132023-10-010963-9969https://hdl.handle.net/2445/217401This study comprehensively characterized the metabolite profiles of six lettuce varieties and established the</p><p>correlation between the elucidated profiles and their antivirulence effects. A total of 195 metabolites were annotated</p><p>using LC-QTOF-MS/MS metabolomics assisted by molecular networking and integrated with chemometrics.</p><p>Red varieties (red longifolia and lolla rosa) demonstrated higher chlorogenic and chicoric acids</p><p>suggesting their antioxidant properties. In parallel, amino acids and disaccharides were enriched in romaine</p><p>longifolia rationalizing its palatable taste and nutritional potential, while crispa, capitata, and lolla bionda presented</p><p>a high β-carboline alkaloid content. The antibacterial and antihemolytic potential of all varieties against</p><p>methicillin-sensitive and methicillin-resistant Staphylococcus aureus was assessed and validated by prominent</p><p>downregulation of α-hemolysin transcriptional levels in both strains. Moreover, correlation analysis revealed</p><p>sesquiterpenes, β-carboline alkaloids, amino acids, and oxy-fatty acids as the main bioactives. Results emphasize</p><p>lettuce significance as a functional food and nutraceutical source, and highlight varieties naturally rich in antibacterial agents to adapt breeding programs.16 p.application/pdfengcc-by-nc-nd (c) Otify et al.., 2023http://creativecommons.org/licenses/by-nc-nd/4.0/MetabolòmicaCromatografiaMetabolomicsChromatographyinfo:eu-repo/semantics/article7381862025-01-13info:eu-repo/semantics/openAccess