Jardí Ripoll, MercèFàbregas, PereSagarra Tió, MaríaPérez Lucena, María JoséFélez, Jordi2018-11-292018-11-292012https://hdl.handle.net/2445/126561The NB4 promyelocytic cell line exhibits many of the characteristics of acute promyelocytic leukemia blast cells, including the translocation (15 : 17) that fuses the PML gene on chromosome 15 to the RAR alpha gene on chromosome 17. These cells have a very high fibrinolytic capacity. In addition to a high secretion of urokinase, NB4 cells exhibit a 10-fold higher plasminogen binding capacity compared with other leukemic cell lines. When tissue-type plasminogen activator was added to acid-treated cells, plasmin generation was 20-26-fold higher than that generated by U937 cells or peripheral blood neutrophils, respectively. We found that plasminogen bound to these cells can be detected by fluorescence-activated cell sorting using an antiplasminogen monoclonal antibody that specifically reacts with this antigen when it is bound to cell surfaces. All-trans retinoid acid treatment of NB4 cells markedly decreased the binding of this monoclonal antibody. This cell line constitutes a unique model to explore plasminogen binding and activation on cell surfaces that can be modulated by all-trans retinoid acid treatment.7 p.application/pdfengcc by (c) Jardí et al., 2012http://creativecommons.org/licenses/by/3.0/es/LeucèmiaCromosomes humansLeukemiaHuman chromosomesinfo:eu-repo/semantics/article2018-07-24info:eu-repo/semantics/openAccess23118518