Ciruela Alférez, FranciscoEscriche, MarisolBurgueño, JavierAngulo, EsterCasadó, VicentSoloviev, Mikhail M.Canela Campos, Enric I. (Enric Isidre), 1949-Mallol Montero, JosefaChan, Wai-YeeLluís i Biset, CarmeMcIlhinney, R. A. JeffreyFranco Fernández, Rafael2018-05-092018-05-092001-050021-9258https://hdl.handle.net/2445/122235Recently, evidence has emerged that seven transmembrane G protein-coupled receptors may be present as homo- and heteromers in the plasma membrane. Here we describe a new molecular and functional interaction between two functionally unrelated types of G protein-coupled receptors, namely the metabotropic glutamate type 1alpha (mGlu(1alpha) receptor) and the adenosine A1 receptors in cerebellum, primary cortical neurons, and heterologous transfected cells. Co-immunoprecipitation experiments showed a close and subtype-specific interaction between mGlu(1alpha) and A1 receptors in both rat cerebellar synaptosomes and co-transfected HEK-293 cells. By using transiently transfected HEK-293 cells a synergy between mGlu(1alpha) and A1 receptors in receptor-evoked [Ca(2+)](i) signaling has been shown. In primary cultures of cortical neurons we observed a high degree of co-localization of the two receptors, and excitotoxicity experiments in these cultures also indicate that mGlu(1alpha) and A1 receptors are functionally related. Our results provide a molecular basis for adenosine/glutamate receptors cross-talk and open new perspectives for the development of novel agents to treat neuropsychiatric disorders in which abnormal glutamatergic neurotransmission is involved.8 p.application/pdfeng(c) American Society for Biochemistry and Molecular Biology, 2001AdenosinaNeurotransmissióÀcid glutàmicAdenosineNeural transmissionGlutamic acidinfo:eu-repo/semantics/article1801142018-05-09info:eu-repo/semantics/openAccess