Rezvani, MaryamManca, Maria LetiziaCaddeo, CarlaEscribano Ferrer, ElviraCarbone, ClaudiaPeris, José EstebanUsach, IrisDiez-Sales, OctavioFadda, Anna MariaManconi, Maria2021-02-112021-02-1120191999-4923https://hdl.handle.net/2445/173852The present study aimed at developing a new vesicular formulation capable of promoting the protective effect of ascorbic acid and tocopherol against intestinal oxidative stress damage, and their efficacy in intestinal wound healing upon oral administration. A pH-dependent copolymer (Eudragit® L100), a water-soluble prebiotic fibre (Nutriose® FM06), a phospholipid mixture (Lipoid S75), and two natural antioxidants (ascorbic acid and tocopherol) were combined to fabricate eudragit-nutriosomes by a simple, solvent-free procedure. The vesicles were spherical and oligolamellar, with some multicompartment structures in Eudragit-nutriosomes, small in size (~100 nm), with highly negative zeta potential. The effect of Eudragit® and Nutriose® on the stability on storage and in simulated gastrointestinal fluids were confirmed by the Turbiscan® technology and in vitro studies, respectively. Eudragit-nutriosomes exhibited a protective effect against H2O2-induced oxidative stress, and a proliferative effect in Caco-2 cells, as they provided the closure of the scratched area after 96 h of incubation. Keywords: Eudragit, Nutriose, phospholipid vesicles, antioxidant, intestinal wound healing10 p.application/pdfengcc-by (c) Rezvani, Maryam et al., 2019http://creativecommons.org/licenses/by/3.0/esVitamina CVitamina ECicatritzacióIntestinsNeuroimmunologiaVitamin CVitamin EWound healingIntestinesNeuroimmunologyinfo:eu-repo/semantics/article6838022021-02-11info:eu-repo/semantics/openAccess30621127