Planinc, IvoGarcia Canadilla, PatriciaDejea, HectorIlic, IvanaGuasch i Casany, EduardZamora, MonicaCrispi Brillas, FàtimaStampanoni, MarcoMilicic, DavorBijnens, BartBonnin, AnneCikes, Maja2022-06-232022-06-232021-01-072045-2322https://hdl.handle.net/2445/186967Cardiovascular research is in an ongoing quest for a superior imaging method to integrate grossanatomical information with microanatomy, combined with quantifable parameters of cardiac structure. In recent years, synchrotron radiation-based X-ray Phase Contrast Imaging (X-PCI) has been extensively used to characterize soft tissue in detail. The objective was to use X-PCI to comprehensively quantify ischemic remodeling of diferent myocardial structures, from cell to organ level, in a rat model of myocardial infarction. Myocardial infarction-induced remodeling was recreated in a well-established rodent model. Ex vivo rodent hearts were imaged by propagation based X-PCI using two confgurations resulting in 5.8 µm and 0.65 µm efective pixel size images. The acquired datasets were used for a comprehensive assessment of macrostructural changes including the whole heart and vascular tree morphology, and quantifcation of left ventricular myocardial thickness, mass, volume, and organization. On the meso-scale, tissue characteristics were explored and compared with histopathological methods, while microstructural changes were quantifed by segmentation of cardiomyocytes and calculation of cross-sectional areas. Propagation based X-PCI provides detailed visualization and quantifcation of morphological changes on whole organ, tissue, vascular as well as individual cellular level of the ex vivo heart, with a single, non-destructive 3D imaging modality.13 p.application/pdfengcc-by (c) Planinc, Ivo et al., 2021https://creativecommons.org/licenses/by/4.0/Diagnòstic per la imatgeRaigs XAnimalsDiagnostic imagingX-raysAnimalsinfo:eu-repo/semantics/article7217802022-06-23info:eu-repo/semantics/openAccess34234175