Kawana-Tachikawa, AiLlibre, Josep MaríaBravo, IsabelEscrig, RoserMothe, BeatrizPuig, JordiPuertas Castro, Ma. CarmenMartínez Picado, Francisco JavierBlanco, JuliàManzardo, ChristianMiró Meda, José M. (José María), 1956-Iwamoto, AikichiPozniak, Anton L.Gatell, José M.Clotet, Bonaventura, 1953-Brander, Christian2017-04-112017-04-112014-01-271932-6203https://hdl.handle.net/2445/109602BACKGROUND: The effect of maraviroc on the maintenance and the function of HIV-1-specific T cell responses remains unknown. METHODS: Subjects recently infected with HIV-1 were randomized to receive anti-retroviral treatment with or without maraviroc intensification for 48 weeks, and were monitored up to week 60. PBMC and in vitro-expanded T cells were tested for responses to the entire HIV proteome by ELISpot analyses. Intracellular cytokine staining assays were conducted to monitor the (poly)-functionality of HIV-1-specific T cells. Analyses were performed at baseline and week 24 after treatment start, and at week 60 (3 months after maraviroc discontinuation). RESULTS: Maraviroc intensification was associated with a slower decay of virus-specific T cell responses over time compared to the non-intensified regimen in both direct ex-vivo as well as in in-vitro expanded cells. The effector function profiles of virus-specific CD8⁺ T cells were indistinguishable between the two arms and did not change over time between the groups. CONCLUSIONS: Maraviroc did not negatively impact any of the measured parameters, but was rather associated with a prolonged maintenance of HIV-1-specific T cell responses. Maraviroc, in addition to its original effect as viral entry inhibitor, may provide an additional benefit on the maintenance of virus-specific T cells which may be especially important for future viral eradication strategies.11 p.application/pdfengcc-by (c) Kawana-Tachikawa, Ai et al., 2014http://creativecommons.org/licenses/by/3.0/esCèl·lules TVIH (Virus)AntiretroviralsResposta immunitàriaGenètica molecularT cellsHIV (Viruses)Antiretroviral agentsImmune responseMolecular geneticsinfo:eu-repo/semantics/article6415972017-04-11info:eu-repo/semantics/openAccess24475275