Calderón Domínguez, MaríaSebastián Muñoz, DavidFucho Salvador, RaquelWeber Blattes, MinéiaMir Bonnín, Joan FrancescGarcía-Casarrubios, EsterObregón, Maria JesúsZorzano Olarte, AntonioValverde, Ángela M.Serra i Cucurull, DolorsHerrero Rodríguez, Laura2016-07-272016-07-272016-07-201932-6203https://hdl.handle.net/2445/101324The discovery of active brown adipose tissue (BAT) in adult humans and the fact that it is reduced in obese and diabetic patients have put a spotlight on this tissue as a key player in obesity-induced metabolic disorders. BAT regulates energy expenditure through thermogenesis; therefore, harnessing its thermogenic fat-burning power is an attractive therapeutic approach. We aimed to enhance BAT thermogenesis by increasing its fatty acid oxidation (FAO) rate. Thus, we expressed carnitine palmitoyltransferase 1AM (CPT1AM), a permanently active mutant form of CPT1A (the rate-limiting enzyme in FAO), in a rat brown adipocyte (rBA) cell line through adenoviral infection. We found that CPT1AM-expressing rBA have increased FAO, lipolysis, UCP1 protein levels and mitochondrial activity. Additionally, enhanced FAO reduced the palmitate-induced increase in triglyceride content and the expression of obese and inflammatory markers. Thus, CPT1AM-expressing rBA had enhanced fat-burning capacity and improved lipid-induced derangements. This indicates that CPT1AM-mediated increase in brown adipocytes FAO may be a new approach to the treatment of obesity-induced disorders.application/pdfengcc-by (c) Calderon-Dominguez, María et al., 2016http://creativecommons.org/licenses/by/3.0/esCarnitina palmitoïl-transferasa 1Teixit adipósObesitatCarnitine palmitoyltransferase IAdipose tissuesObesityinfo:eu-repo/semantics/article6632892016-07-27info:eu-repo/semantics/openAccess27438137