Torres, PascualAnerillas, CarlosRamírez Núñez, OmarFernàndez, AnnaEncinas, MarioPovedano, MònicaAndrés Benito, PolFerrer, Isidro (Ferrer Abizanda)Ayala, VictòriaPamplona, ReinaldPortero-Otin, Manuel2022-10-252022-10-252022-08-011754-8411https://hdl.handle.net/2445/190166To evaluate senescence mechanisms, including senescence-associated secretory phenotype (SASP), in the motor neuron disease model hSOD1-G93A, we quantified the expression of p16 and p21 and senescence-associated Ji-galactosidase (SA-Ji-gal) in nervous tissue. As SASP markers, we measured the mRNA levels of Il1a , Il6 , Ifna and Ifnb. Furthermore, we explored whether an alteration of alternative splicing is associated with senescence by measuring the Adipor2 cryptic exon inclusion levels, a specific splicing variant repressed by TAR DNA-binding protein (TDP-43; encoded by Tardbp). Transgenic mice showed an atypical senescence profile with high p16 and p21 mRNA and protein in glia, without the canonical increase in SA-Ji-gal activity. Consistent with SASP, there was an increase in Il1a and Il6 expression, associated with increased TNF-R and M-CSF protein levels, with females being partially protected. TDP-43 splicing activity was compromised in this model, and the senolytic drug Navitoclax did not alter the disease progression. This lack of effect was reproduced in vitro , in contrast to dasatinib and quercetin, which diminished p16 and p21. Our findings show a non-canonical profile of senescence biomarkers in the model hSOD1-G93A.11 p.application/pdfengcc by (c) Torres, Pascual et al., 2022http://creativecommons.org/licenses/by/3.0/es/Esclerosi lateral amiotròficaRNAAmyotrophic lateral sclerosisRNAinfo:eu-repo/semantics/article2022-10-20info:eu-repo/semantics/openAccess35916061