Pérez-Rodríguez, Francisco JavierD'Andrea Rodríguez-Vida, LucíaCastellarnau Serra, Montserrat deCostafreda Salvany, M. Isabel (Maria Isabel)Guix Arnau, SusanaRibes Mora, EnricQuer, JosepGregori Font, JosepBosch, AlbertPintó Solé, Rosa María2017-10-132017-10-132016-11-032045-2322https://hdl.handle.net/2445/116589Virus production still is a challenging issue in antigen manufacture, particularly with slow-growing viruses. Deep-sequencing of genomic regions indicative of efficient replication may be used to identify high-fitness minority individuals suppressed by the ensemble of mutants in a virus quasispecies. Molecular breeding of quasispecies containing colonizer individuals, under regimes allowing more than one replicative cycle, is a strategy to select the fittest competitors among the colonizers. A slow-growing cell culture-adapted hepatitis A virus strain was employed as a model for this strategy. Using genomic selection in two regions predictive of efficient translation, the internal ribosome entry site and the VP1-coding region, high-fitness minority colonizer individuals were identified in a population adapted to conditions of artificially-induced cellular transcription shut-off. Molecular breeding of this population with a second one, also adapted to transcription shut-off and showing an overall colonizer phenotype, allowed the selection of a fast-growing population of great biotechnological potential.12 p.application/pdfengcc-by (c) Pérez et al., 2016http://creativecommons.org/licenses/by/3.0/esVirologiaEspècies (Biologia)VirologySpeciesinfo:eu-repo/semantics/article6649892017-10-13info:eu-repo/semantics/openAccess27808108