Llaudó Vallmajor, InésCassis, L.Torras Ambròs, JoanBestard Matamoros, OriolFranquesa, MarcellaCruzado, Josep Ma.Cerezo, GemaCastaño Boldú, EstherPétriz, JordiHerrero Fresneda, ImmaculadaGrinyó Boira, Josep M.Lloberas Blanch, Núria2013-09-172013-09-172012-07-151482-1826https://hdl.handle.net/2445/46026Purpose. P-glycoprotein (Pgp) is a member of the ABC-transporter family that transports substances across cellular membranes acting as an efflux pump extruding drugs out of the cells. Pgp plays a key role on the pharmacokinetics of several dr ugs. Herein, we have studied the effects of immunosuppressants on Pgp function, assessing rhodamine-123 (Rho123) uptake and efflux in different T- cell subsets. Methods. Different immunosuppressants such as Cyclosporine (CsA), Rapamycin (Rapa) and Tacrolimus (Tac) were used to assess the in vitro effect on Pgp function of main T-cell subsets among healthy volunteers. We measured Rho123 upta ke, efflux and kinetic of extrusion in CD4 + and CD8 + subsets by flow cytometry. Antigen-specific memory T-ce ll responses were assessed by measuring T-cell proliferation and cytokine secretion using an allogeneic mixed lymphocyte reaction. Results. Rho123 uptake in groups treated with CsA and CsA+Rapa was signif icantly decreased compared to non-treated group and the other immunosupressants in both T cells subsets. Pgp activity was also reduced in CsA and CsA+Rapa compared to the other immunosupressants but it was only significant in the CsA group for CD8 + subset. Kinetic extrusion of Rho123 by Pgp in all groups was faster in CD8 + T cells. All immunosuppressants and the specific Pgp inhibitor PSC833 diminished antigen-primed T-cell proliferation, especially CD8 + T-cell subset. Conclusions. Our data indicate that small molecules immunosuppressants, especially CsA, inhibit Pgp activity and T-cell function being the CD8 + T cells more susceptible to this effect. These findings support the importance of Pgp when designing combined immunosuppressive regimens.13 p.application/pdfengcc-by-sa (c) Llaudó, I. et al., 2012http://creativecommons.org/licenses/by-sa/3.0/esImmunosupressióTransport biològicProteïnes de membranaImmunosuppressionBiological transportMembrane proteinsinfo:eu-repo/semantics/article6216812013-09-17info:eu-repo/semantics/openAccess