Clapé, CyrielleFritz, VanessaHenriquet, CorinneApparailly, FlorenceFernández Ruiz, Pedro LuisIborra, FrançoisAvancès, ChristopheVillalba, MartinCuline, StéphaneFajas, Lluis2013-06-062013-06-062009-10-261932-6203https://hdl.handle.net/2445/44087Abstract Background: Micro RNAs are small, non-coding, single-stranded RNAs that negatively regulate gene expression at the post-transcriptional level. Since miR-143 was found to be down-regulated in prostate cancer cells, we wanted to analyze its expression in human prostate cancer, and test the ability of miR-43 to arrest prostate cancer cell growth in vitro and in vivo. Results: Expression of miR-143 was analyzed in human prostate cancers by quantitative PCR, and by in situ hybridization. miR-143 was introduced in cancer cells in vivo by electroporation. Bioinformatics analysis and luciferase-based assays were used to determine miR-143 targets. We show in this study that miR-143 levels are inversely correlated with advanced stages of prostate cancer. Rescue of miR-143 expression in cancer cells results in the arrest of cell proliferation and the abrogation of tumor growth in mice. Furthermore, we show that the effects of miR-143 are mediated, at least in part by the inhibition of extracellular signal-regulated kinase-5 (ERK5) activity. We show here that ERK5 is a miR-143 target in prostate cancer. Conclusions: miR-143 is as a new target for prostate cancer treatment.8 p.application/pdfengcc-by (c) Clapé, C. et al., 2009http://creativecommons.org/licenses/by/3.0/esMicro RNAsCàncer de pròstataBioinformàticaMicroRNAsProstate cancerBioinformaticsinfo:eu-repo/semantics/article5869522013-06-06info:eu-repo/semantics/openAccess19855844