Itarte, MartaBofill Mas, SilviaMartínez-Puchol, SandraTorrell, HelenaCeretó, AdriàCarrasco, Marina (Carrasco Pérez)Forés, EvaCanela, NúriaGironès Llop, RosinaRusiñol, Marta2022-03-162023-12-312021-122468-5844https://hdl.handle.net/2445/184139SARS-CoV-2 variants are emerging worldwide, and monitoring them is key in providing early warnings. Here, we summarize the different analytical approaches currently used to study the dissemination of SARS-CoV-2 variants in wastewater and discuss their advantages and disadvantages. We also provide preliminary results of two sensitive and cost-effective approaches: variant-specific reverse transcription-nested PCR assays and a nonvariant-specific amplicon deep sequencing strategy that targets three key regions of the viral spike protein. Next-generation sequencing approaches enable the simultaneous detection of signature mutations of different variants of concern in a single assay and may be the best option to explore the real picture at a particular time. Targeted PCR approaches focused on specific signature mutations will need continuous updating but are sensitive and cost-effective.20 p.application/pdfengcc-by-nc-nd (c) Elsevier B.V., 2021https://creativecommons.org/licenses/by-nc-nd/4.0/SARS-CoV-2Aigües residualsSARS-CoV-2Sewageinfo:eu-repo/semantics/article7203662022-03-16info:eu-repo/semantics/openAccess