Fernandez, AntonioKielland, NicolaMakda, AshraffCarragher, NeilGonzález-García, M. CarmenEspinar-Barranco, LauraGonzález-Vera, Juan AntonioOrte, ÁngelLavilla Grífols, RodolfoVendrell, Marc2022-12-152022-12-152022-08-282227-9059https://hdl.handle.net/2445/191606We report a platform combining multicomponent reaction synthesis and automated cell-based screening to develop biocompatible NIR-BODIPY fluorophores. From a library of over 60 fluorophores, we optimised compound NIRBD-62c as a multimodal probe with suitable properties for STED super-resolution and fluorescence lifetime imaging. Furthermore, we employed NIRBD-62c for imaging trafficking inside cells and to examine how pharmacological inhibitors can alter the vesicular traffic between intracellular compartments and the plasma membrane.application/pdfengcc-by (c) Fernandez, Antonio et al., 2022https://creativecommons.org/licenses/by/4.0/Química farmacèuticaSíntesi orgànicaPharmaceutical chemistryOrganic synthesisinfo:eu-repo/semantics/article7244842022-12-15info:eu-repo/semantics/openAccess