Linares Galiana, IsabelBerenguer Francés, Miguel ÁngelCañas Cortés, RutPujol Canadell, MonicaComas Antón, SilviaMartínez, EvelynLaplana, MariaPérez Montero, HéctorPla Farnós, María JesúsNavarro Martín, ArturoNuñez, MiriamBoth, BrigitteGuedea Edo, Ferran2021-03-012021-03-012021-010449-3060https://hdl.handle.net/2445/174434A detailed understanding of the interactions and the best dose-fractionation scheme of radiation to maximize antitumor immunity have not been fully established. In this study, the effect on the host immune system of a single dose of 20 Gy through intraoperative radiation therapy (IORT) on the surgical bed in low-risk breast cancer patients undergoing conserving breast cancer has been assessed. Peripheral blood samples from 13 patients were collected preoperatively and at 48 h and 3 and 10 weeks after the administration of radiation. We performed a flow cytometry analysis for lymphocyte subpopulations, natural killer cells (NK), regulatory T cells (Treg) and myeloid-derived suppressor cells (MDSCs). We observed that the subpopulation of NK CD56+high CD16+ increased significantly at 3 weeks after IORT (0.30-0.42%, P < 0.001), while no changes were found in immunosuppressive profile, CD4+CD25+Foxp3+Helios+ Treg cells, granulocytic MDSCs (G-MDSCs) and monocytic MDSCs (Mo-MDSCs). A single dose of IORT may be an effective approach to improve antitumor immunity based on the increase in NK cells and the non-stimulation of immunosuppressive cells involved in immune escape. These findings support future combinations of IORT with immunotherapy, if they are confirmed in a large cohort of breast cancer patients.9 p.application/pdfengcc-by (c) Linares Galiana, Isabel et al., 2021http://creativecommons.org/licenses/by/3.0/esCàncer de mamaRadioteràpiaImmunoteràpiaBreast cancerRadiotherapyImmunotheraphyinfo:eu-repo/semantics/article7070702021-03-01info:eu-repo/semantics/openAccess33006364