Rafecas Jorba, ImmaculadaEsteve Ràfols, MontserratFernández López, José AntonioRemesar Betlloch, XavierAlemany, Marià, 1946-2012-02-242012-02-2419940966-0844https://hdl.handle.net/2445/22229The amino acid composition of the protein from three strains of rat (Wistar, Zucker lean and Zucker obese), subjected to reference and high-fat diets has been used to determine the mean empirical formula, molecular weight and N content of whole-rat protein. The combined whole protein of the rat was uniform for the six experimental groups, containing an estimate of 17.3% N and a mean aminoacyl residue molecular weight of 103.7. This suggests that the appropriate protein factor for the calculation of rat protein from its N content should be 5.77 instead of the classical 6.25. In addition, an estimate of the size of the non-protein N mass in the whole rat gave a figure in the range of 5.5 % of all N. The combination of the two calculations gives a protein factor of 5.5 for the conversion of total N into rat protein.11 p.application/pdfeng(c) The Nutrition Society, 1994Rates (Animals de laboratori)Proteïnes dels alimentsNitrogen conversion factor (6.25)Protein analysisProtein nitrogenRatWhole-rat protein content estimation: applicability of the Nx6.25 factorinfo:eu-repo/semantics/article171930info:eu-repo/semantics/openAccess