Camarero, NúriaTrapero, AnaPérez Jiménez, AriadnaMacia, EricGomila Juaneda, AlexandreGorostiza Langa, Pablo IgnacioNevola, LauraLlobet Berenguer, Artur, 1972-Llebaria Soldevila, AmadeuHernando, JordiGiralt Lledó, Ernest2021-01-252021-01-2520202041-6520https://hdl.handle.net/2445/173364The spatiotemporal control of cellular dynamic processes has great fundamental interest but lacks versatile molecular tools. Dynamin is a key protein in endocytosis and an appealing target to manipulate cell trafficking using patterns of light. We have developed the first photoswitchable small-molecule inhibitors of endocytosis (dynazos), by a stepwise design of the photochromic and pharmacological properties of dynasore, a dynamin inhibitor. We have characterized their photochromism with UV-visible and transient absorption spectroscopy and their biological activity using fluorescence microscopies and flow cytometry. Dynazos are water-soluble, cell permeable, and photostable, and enable fast, single- wavelength photoswitchable inhibition of clathrin-mediated endocytosis at micromolar concentration.8 p.application/pdfeng(c) Camarero, Núria et al., 2020Interacció cel·lularEspectroscòpia molecularCell interactionMolecular spectroscopyinfo:eu-repo/semantics/article7039572021-01-25info:eu-repo/semantics/openAccess33016959