Diaz Riascos, Zamira VanessaGinestà, Mireia M.Fabregat Prous, JoanSerrano Piñol, M. TeresaBusquets Barenys, JuliBuscail, LouisCordelier, PierreCapellá, G. (Gabriel)2020-10-192020-10-192019-09-062162-2531https://hdl.handle.net/2445/171332MicroRNAs from the miR-200 family are commonly associated with the inhibition of the metastatic potential of cancer cells, following inhibition of ZEB transcription factors expression and epithelial-to-mesenchymal transition. However, previous studies performed in pancreatic adenocarcinoma revealed a more complex picture challenging this canonical model. To gain better insights into the role of miR-200 family members in this disease, we analyzed the expression of miR-200a, miR- 200b, miR-200c, miR-141, miR-429, and miR-205, and ZEB1, ZEB2, and CDH1 in pancreatic tumors and matching normal adjacent parenchyma and patient-derived xenografts. We found that miR-200a, miR-429, and miR-205 are frequently overex- pressed in pancreatic tumors, whereas CDH1 is downregulated, and ZEB1 and ZEB2 levels remain unchanged. Furthermore, we measured a positive correlation between miR-200 family mem- bers and CDH1 expression, and a negative correlation between ZEB1 and miR-200c, miR-141, and miR-205 expression, respec- tively. Interestingly, we identified significant changes in expres- sion of epithelial-to-mesenchymal transition regulators and miR-200 members in patient-derived xenografts. Lastly, func- tional studies revealed that miR-141 and miR-429 inhibit the tumorigenic potential of pancreatic cancer cells. Taken together, this comprehensive analysis strongly suggests that miRNAs from the miR-200 family, and in particular miR-429, may act as a tu- mor suppressor gene in pancreatic cancer.13 p.application/pdfengcc-by-nc-nd (c) Diaz Riascos, Zamira Vanessa et al., 2019http://creativecommons.org/licenses/by-nc-nd/3.0/esCàncer de pàncreesMetàstasiMicro RNAsPancreas cancerMetastasisMicroRNAsinfo:eu-repo/semantics/article7035042020-10-19info:eu-repo/semantics/openAccess31336236