Sarri Plans, ElisabetMartínez Rodríguez, Susana EvaJiménez, NuriaClaro Izaguirre, EnriqueMérida, IsabelBurger, Koert N. J.Egea Guri, GustavoFernández Ulibarri, InésVilella, MontserratLázaro Diégez, Francisco2012-04-272012-04-2720071059-1524https://hdl.handle.net/2445/24655Diacylglycerol is necessary for trans-Golgi network (TGN) to cell surface transport, but its functional relevance in the early secretory pathway is unclear. Although depletion of diacylglycerol did not affect ER-to-Golgi transport, it led to a redistribution of the KDEL receptor to the Golgi, indicating that Golgi-to-ER transport was perturbed. Electron microscopy revealed an accumulation of COPI-coated membrane profiles close to the Golgi cisternae. Electron tomography showed that the majority of these membrane profiles originate from coated buds, indicating a block in membrane fission. Under these conditions the Golgi-associated pool of ARFGAP1 was reduced, but there was no effect on the binding of coatomer or the membrane fission protein CtBP3/BARS to the Golgi. The addition of 1,2-dioctanoyl-sn-glycerol or the diacylglycerol analogue phorbol 12,13-dibutyrate reversed the effects of endogenous diacylglycerol depletion. Our findings implicate diacylglycerol in the retrograde transport of proteins from Golgi to the ER and suggest that it plays a critical role at a late stage of COPI vesicle formation.14 p.application/pdfengcc-by-nc-sa (c) Fernández-Ulibarri et al., 2007http://creativecommons.org/licenses/by-nc-sa/3.0/Aparell de GolgiReticle endoplasmàticProteïnesCitologiaTransport biològicGolgi apparatusEndoplasmic reticulumProteinsCytologyBiological transportinfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1091/mbc.E07-04-0334552867info:eu-repo/semantics/openAccess17567948