Gupta, HimanshuMacete, Eusebio VíctorBulo, HelderSalvador, CrizolgoWarsame, MarianCarvalho, EvaMenard, DidierRingwald, PascalBassat Orellana, QuiqueEnosse, SoniaMayor Aparicio, Alfredo Gabriel2018-01-172018-01-172018-011080-6040https://hdl.handle.net/2445/119078One of the fundamental steps toward malaria control is the use of antimalarial drugs. The success of antimalarial treatment can be affected by the presence of drug-resistant populations of Plasmodium falciparum. To assess resistance, we used molecular methods to examine 351 P. falciparum isolates collected from 4 sentinel sites in Mozambique for K13, pfmdr1, pfcrt, and pfdhps polymorphisms and for plasmepsin2 (pfpm2) and pfmdr1 copy numbers. We found multiple copies of pfpm2 in 1.1% of isolates. All isolates carried K13 wild-type alleles (3D7-like), except 4 novel polymorphisms (Leu619Leu, Phe656Ile, Val666Val, Gly690Gly). Prevalence of isolates with pfcrt mutant (K76T) allele was low (2.3%). Prevalence of isolates with pfdhps mutant alleles (A437G and K540E) was >80%, indicating persistence of sulfadoxine/pyrimethamine resistance; however, markers of artemisinin were absent, and markers of piperaquine resistance were low. Piperaquine resistance isolates may spread in Mozambique as dihydroartemisinin/piperaquine drug pressure increases.9 p.application/pdfengDomini públic / Public domainhttp://creativecommons.org/publicdomain/mark/1.0/Plasmodium falciparumMoçambicPlasmodium falciparumMozambiqueinfo:eu-repo/semantics/article2017-12-27info:eu-repo/semantics/openAccess29260689