Arias Alpízar, KevinSánchez-Cano, A.Prat-Trunas, J.Serna, E.Alonso Casanovas, OscarSulleiro, E.Sánchez-Montalvá, AdriánDiéguez Barrientos, ÀngelBaldrich, E.2026-01-162026-01-162022-11-010956-5663https://hdl.handle.net/2445/225668A point-of-care (POC) device is reported for highly sensitive and selective detection of Plasmodium falciparum lactate dehydrogenase (Pf-LDH), a biomarker of malaria infection, based on a single-step magneto-immunoassay, a single-use microfluidic paper device and a customized hand-held fluorescence reader. The single-step magneto-immunoassay consists in a single 5-min incubation of immuno-modified magnetic particles (c-MAb-MPs), biotinylated detection antibody (bd-MAb), and an enzymatic signal amplifier (Poly-HRP). After on-chip MP concentration and washing, signal generation is achieved by adding a fluorescent enzymatic substrate (QuantaRed). Fluorescence signal is measured using a low-cost customized, portable, and sensible fluorescent detector. The POC affords quantitative Pf-LDH detection in <20 min, with a detection limit of 0.92 ng mL−1 (equivalent to 4.6 parasites μL−1). Furthermore, Pf-LDH quantitation in clinical samples correlates with that provided by the reference ELISA, is more sensitive than a commercial rapid diagnostic test (RDT) and entails little user intervention. These results show that fluorescent paper-based microfluidic devices can be exploited to simplify magneto-immunoassay handling, taking this type of test closer to the requirements of POC testing.8 p.application/pdfengcc-by-nc-nd (c) Elsevier B.V., 2022http://creativecommons.org/licenses/by-nc-nd/4.0/Plasmodium falciparumMalàriaDiagnòsticPlasmodium falciparumMalariaDiagnosisinfo:eu-repo/semantics/article7245382026-01-16info:eu-repo/semantics/openAccess