Easy introduction of maleimides at different positions of oligonucleotide chains for conjugation purposes

Sánchez-Moya, AlbertPedroso Muller, EnriqueGrandas Sagarra, Anna2014-02-062014-02-0620121477-0520https://hdl.handle.net/2445/49472[2,5-Dimethylfuran]-protected maleimides were placed at both internal positions and the 3'-end of oligonucleotides making use of solid-phase synthesis procedures. A new phosphoramidite derivative and a new solid support incorporating the protected maleimide moiety were prepared for this purpose. In all cases maleimide deprotection (retro-Diels-Alder reaction) followed by reaction with thiol-containing compounds afforded the target conjugate.6 p.application/pdfeng(c) Sánchez, Albert et al., 2012BiotecnologiaÀcids nucleicsEspectrometria de massesNucleòtidsCompostos heterocíclicsBiotechnologyNucleic acidsMass spectrometryNucleotidesHeterocyclic compoundsEasy introduction of maleimides at different positions of oligonucleotide chains for conjugation purposesinfo:eu-repo/semantics/article6169252014-02-06info:eu-repo/semantics/openAccess23007699