García García de Paredes, AnaManicardi, NicoloTellez, LuisIbañez, LuisRoyo, FelixBermejo, JavierBlanco, CarolinaFondevila Campo, ConstantinoFernández Lanza, ValGarcía Bermejo, LauraFalcón Pérez, Juan ManuelBañares, RafaelGracia Sancho, JordiAlbillos, Agustín2021-06-012021-06-012020-12-022471-254Xhttps://hdl.handle.net/2445/177844Noninvasive staging of decompensated cirrhosis is an unmet clinical need. The aims of this study were to characterize and validate a novel microRNA (miRNA) signature to stage decompensated cirrhosis and predict the portal pressure and systolic cardiac response to nonselective beta-blockers (NSBBs). Serum samples from patients with decompensated cirrhosis (n = 36) and healthy controls (n = 36) were tested for a novel signature of five miRNAs (miR-452-5p, miR-429, miR-885-5p, miR-181b-5p, and miR-122-5p) identified in the secretome of primary human hepatocytes and for three miRNAs (miR-192-5p, miR-34a-5p, and miR-29a-5p) previously discovered as biomarkers of chronic liver disease. All patients had ascites, which was refractory in 18 (50%), and were placed on NSBBs for variceal bleeding prophylaxis. In all patients, serum miRNAs, hepatic venous pressure gradient, and an echocardiogram study were performed before and 1 month after NSBBs. Patients with cirrhosis had lower serum levels of miR-429, miR-885-5p, miR-181b-5p, miR-122-5p, miR-192-5p, and miR-29a-5p (P < 0.05). Baseline serum miR-452-5p and miR-429 levels were lower in NSBB responders (P = 0.006). miR-181b-5p levels were greater in refractory ascites than in diuretic-sensitive ascites (P = 0.008) and correlated with serum creatinine. miR-452-5p and miR-885-5p were inversely correlated with baseline systemic vascular resistance (ρ = −0.46, P = 0.007; and ρ = −0.41, P = 0.01, respectively) and with diminished systolic contractility (ρ = −0.55, P = 0.02; and ρ = −0.55, P = 0.02, respectively) in patients with refractory ascites after NSBBs. Conclusion: Analysis of a miRNA signature in serum discriminates between patients with decompensated cirrhosis who show more severe systemic circulatory dysfunction and compromised systolic function after beta-blockade and those more likely to benefit from NSBBs.14 p.application/pdfengcc-by (c) García García de Paredes, Ana et al., 2020https://creativecommons.org/licenses/by/4.0/Cirrosi hepàticaMicro RNAsMarcadors bioquímicsHepatic cirrhosisMicroRNAsBiochemical markersinfo:eu-repo/semantics/article7054872021-06-01info:eu-repo/semantics/openAccess33553977