Barbosa, Thallysson CarvalhoDias Nascimento, Lívia ÉvenBani, CristianeAlmeida, TalineNery, MarceloSantos, Rafael Silvade Oliveira Menezes, Luana RenyelleZielinska, AleksandraFernandes, Ana RitaCordeiro Cardoso, JulianaJäguer, AlessandroJäguer, EliezerSánchez-López, E. (Elena)Nalone, LucianaBarbosa Souto, ElianaSeverino, Patrícia2020-06-232020-06-232019-09-222305-6304https://hdl.handle.net/2445/166524The objective of this work was to characterize the toxicological profile of a newly developed sunscreen formulation based on polymeric nanocapsules (NCs) loading benzophenone-3 (BZP3). NCs composed of poly(ε-caprolactone) carrot oil and Pluronic® F68 were produced by emulsification-diffusion method. Their mean particle size (Z-Ave) ranged from 280 to 420 nm, polydispersity index (PDI) was below 0.37, while zeta potential (ZP) reached about |+11 mV|. No cytotoxic effects were observed in L929 fibroblast cell line for the blank (i.e., non-loaded) NCs and BZP3-loaded NCs (BZP3-NCs). The semi-solid sunscreen formulation was stable over time (centrifugation testing) and exhibited non-Newtonian pseudoplastic behavior, which is typical of products for topical application onto the skin. The sun protection factor (SPF) value reached 8.84, when incorporating BZP3-NCs (SPF of 8.64) into the semi-solid formulation. A synergistic effect was also observed when combining the formulation ingredients of nanocapsules, i.e., SPF of carrot oil was 6.82, blank NCs was 6.84, and BZP3-loaded NCs was 8.64. From the hen's egg-chorioallantoic membrane test (HET-CAM) test, the non-irritation profile of the developed formulations could also be confirmed. The obtained results show a promising use of poly(ε-caprolactone) nanocapsules to be loaded with lipophilic sunscreens as benzophenone-312 p.application/pdfengcc-by (c) Barbosa, Thallysson Carvalho et al., 2019http://creativecommons.org/licenses/by/3.0/esToxicologia experimentalFarmacocinèticaFarmacologiaProtectors solarsEmulsionsExperimental toxicologyPharmacokineticsPharmacologySunscreens (Cosmetics)Emulsionsinfo:eu-repo/semantics/article7013062020-06-23info:eu-repo/semantics/openAccess31546707