Pont Villanueva, LauraMarin, GemmaVergara-Barberán, MariaGagliardi, Leonardo G.Sanz Nebot, María VictoriaHerrero-Martínez, J. M.Benavente Moreno, Fernando J. (Julián)2020-05-252022-04-182020-04-180021-9673https://hdl.handle.net/2445/162210In this study, polymeric monoliths with gold nanoparticles (AuNP@monolith) were investigated as microcartridges for the analysis of protein biomarkers by on-line solid-phase extraction capillary electrophoresis-mass spectrometry (SPE-CE-MS). "Plug-and-play" microcartridges (7 mm) were prepared from a glycidyl methacrylate (GMA)-based monolithic capillary column (5 cm x 250 µm i.d.), which was modified with ammonia and subsequently functionalized with gold nanoparticles (AuNPs). The performance of these novel microcartridges was evaluated with human transthyretin (TTR), which is a protein related to different types of familial amyloidotic polyneuropathies (FAP). Protein retention depended on the isoelectric point of the protein (TTR pI~5.4) and elution was achieved with a basic phosphate solution. Under the optimized conditions, limits of detection (LODs) for TTR by AuNP@monolith-SPE-CE-MS were 50 times lower than by CE-MS (5 vs 250 mg L−1, with an ion trap (IT) mass spectrometer). The sensitivity enhancement was similar compared to SPE-CE-MS using immunoaffinity (IA) microcartridges with intact antibodies against TTR. Linearity, repeatability in migration times and peak areas, reusability, reproducibility and application to serum samples were also evaluated34 p.application/pdfengcc-by-nc-nd (c) Elsevier B.V., 2020http://creativecommons.org/licenses/by-nc-nd/3.0/esElectroforesi capil·larOrNanopartículesEspectrometria de massesCapillary electrophoresisGoldNanoparticlesMass spectrometryinfo:eu-repo/semantics/article7009942020-05-25info:eu-repo/semantics/openAccess